Differential surface phenotype and context-dependent reactivity of functionally diverse NKT cells

G Cameron; DI Godfrey

Journal article

Differential surface phenotype and context-dependent reactivity of functionally diverse NKT cells

G Cameron, DI Godfrey

Immunology and Cell Biology | WILEY | Published : 2018

DOI: 10.1111/imcb.12034

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Abstract

Natural Killer T (NKT) cells are a functionally diverse population that recognizes lipid-based antigens in association with the antigen-presenting molecule CD1d. Here, we define a technique to separate the functionally distinct thymic NKT1, NKT2 and NKT17 cell subsets by their surface expression of CD278 (ICOS) and the activation-associated glycoform of CD43, enabling the investigation of subset-specific effector-functions. We report that all three subsets express the transcription factor GATA-3 and the potential to produce IL-4 and IL-10 following activation. This questions the notion that NKT2 cells are the predominant source of IL-4 within the NKT cell pool, and suggests that IL-10-produc..

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University of Melbourne Researchers

Garth Cameron Author

Dale Godfrey Author

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Funding Acknowledgements

We are grateful to Dr Paul Savage (Brigham Young University, UT, USA) for providing alpha-GalCer analogue PBS-44. We thank staff from the Flow Cytometry facility at the Department of Microbiology and Immunology at The University of Melbourne. This work was supported by the National Health and Medical Research Council of Australia (NHMRC; 1013667, 1063587, 1113293) and the Australian Research Council (ARC; CE140100011). DIG is supported by an NHMRC Senior Principal Research Fellowship (1020770 and 1117766).